Vibrational spectroscopy as a powerful tool to follow-up immunoadsorption therapy treatment of dilated cardiomyopathy – a case report

in: Analyst (2020)
Huang, Jing; Ramoji, Anuradha; Guo, Shuxia; Bocklitz, Thomas W.; Boivin-Jahns, Valérie; Möller, Jan; Kiehntopf, Michael; Noutsias, Michel; Popp, Jürgen; Neugebauer, Ute
Dilated cardiomyopathy (DCM) is a leading cardiomyopathy entity and is the leading reason for heart transplantation. Due to high etiologic and genetic heterogeneity of the pathologies, different therapeutic treatment strategies are available and succeed in terms of different treatments. Immunoadsorption (IA) therapy removes the circulating anticardiac antibodies and improves the left ventricular function in substantial propotion of DCM patients. Powerful, non-invasive analytical tools are highly desired to investigate the efficiency and success of IA therapy. In this contribution, we followed changes of a female DCM patient undergoing IA therapy at different treatment time-points in a label-free, non-invasive manner directly from blood samples (plasma and serum) on the basis of vibrational spectroscopy (Raman scattering and IR absorption). Chemometric methods including dimension reduction and statistical modeling were used to interpret spectral data. Different IA treatment time points can be differentiated with a high accuracy. Removal of antibodies of IgG group during IA therapy and their restoration was reflected in both Raman and FT-IR spectra. Relative changes in the spectral bands assigned to IgG correlated well with ELISA measurement of total IgG. The successful clinical treatment was accompanied by the spectral differences between vibrational spectra measured at initial disease state and 11 months after the IA treatment. Long-term follow-up of the patient reveals stabilization of the health state after therapy. Noteworthy, the treatment time points were distinguished with a better accuracy using spectra from plasma than those from serum samples, might indicate the involvement of corresponding proteins in the coagulation. Vibrational spectroscopy is a powerful tool to follow-up the treatment success of IA therapy in DCM disorder.

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