Chip-based duplex real-time PCR for water quality monitoring concerning Legionella pneumophila and Legionella spp.

in: Water and Environmental Journal (2021)
Reuter, Cornelia; Hentschel, Stefanie; Breitenstein, Antje; Heinrich, Eileen; Aehlig, Oliver; Henkel, Thomas; Csáki, Andrea; Fritzsche, Wolfgang
Based on biomolecular methods, rapid and selective identification of human pathogenic water organisms becomes an important issue. Legionella spp., are pathogenic water bacteria with worldwide significance. Prevalent detection methods for these microorganisms are time and/or cost intensive. We describe a detection setup and relating DNA assay. A miniaturized real-time polymerase chain reaction (real-time PCR) for direct on-line discrimination of Legionella pneumophila and Legionella spp. was established and integrated into a real-time PCR-chipsystem. The PCR-chip device combines a temperature controlling unit and a fluorescence intensity measurement. It was designed to achieve rapid amplification, using an approach of real-time fluorescence read out with the intercalating dye EvaGreen® and melting curve analysis, without requiring multiple probes. The presented results exhibit reproducibility and good sensitivity, showing that the setup is suitable for robust, rapid and cost-efficient detection and monitoring of a variety of Legionella urban water samples.

Third party cookies & scripts

This site uses cookies. For optimal performance, smooth social media and promotional use, it is recommended that you agree to third party cookies and scripts. This may involve sharing information about your use of the third-party social media, advertising and analytics website.
For more information, see privacy policy and imprint.
Which cookies & scripts and the associated processing of your personal data do you agree with?

You can change your preferences anytime by visiting privacy policy.