Resolution in super-resolution microscopy — definition, trade-offs and perspectives

in: Nature Reviews Molecular Cell Biology (2024)
Prakash, Kirti; Baddeley, David; Eggeling, Christian; Fiolka, Reto; Heintzmann, Rainer; Manley, Suliana; Radenovic, Aleksandra; Smith, Carlas; Shroff, Hari; Schermelleh, Lothar
Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution and common trade-ofs, such as labelling and post-processing, aiming to clarify them for biologists and facilitate deeper understanding and best use of SRM.

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