Interferometric Scattering (iSCAT) Microscopy: studies of biological membrane dynamics

in: SPIE Proceedings (2018)
Reina, Francesco; Galiani, Silvia; Shrestha, Dilip; Sezgin, Erdinc; Cole, Daniel; Lagerholm, B. Christoffer; Kukura, Philip; Eggeling, Christian
The study of the organization and dynamics of molecules in model and cellular membranes is an important topic in contemporary biophysics. Imaging and single particle tracking in this particular field, however, proves particularly demanding, as it requires simultaneously high spatio-temporal resolution and high signal-to-noise ratios. A remedy to this challenge might be Interferometric Scattering (iSCAT) microscopy, due to its fast sampling rates, label-free imaging capabilities and, most importantly, tuneable signal level output. Here we report our recent advances in the imaging and molecular tracking on phase-separated model membrane systems and live-cell membranes using this technique.

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