Combined fiber probe for fluorescence lifetime and Raman spectroscopy
in: Analytical and Bioanalytical Chemistry (2015)
In this contribution we present a dual modality fiber optic probe combining fluorescence lifetime imaging (FLIm) and Raman spectroscopy for in vivo endoscopic applications. The presented EmVision LLC multispectroscopy probe enables efficient excitation and collection of fluorescence lifetime signals for FLIm in the UV/visible wavelength region, as well as of Raman spectra in the near IR for simultaneous Raman/FLIm imaging. The probe has been characterized for its lateral resolution and distance dependency of the Raman and FLIm signals. In addition, the feasibility of the probe for in vivo FLIm and Raman spectral characterization of tissue was demonstrated.